Regulatory

Part:BBa_K2560304:Design

Designed by: Benjamin Daniel   Group: iGEM18_Marburg   (2018-10-03)


3-HP Sensor. This part is a PhytoBrick variant


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1108
    Illegal NotI site found at 279
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 263
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We also amplified 200 bp upstream of the gene, so that all parts of the unannotated promoter were also in our sequence.


Source

Amplified from genomic DNA of Pseudomonas putida KT2440.

References